In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_3 ( 2013-03-01), p. 959-964
Abstract:
A sulfate-reducing bacterium, designated JHA1 T , was isolated from a permanently cold marine sediment sampled in an Artic fjord on the north-west coast of Svalbard. The isolate was originally enriched at 4 °C in a highly diluted liquid culture amended with hydrogen and sulfate. Strain JHA1 T was a psychrophile, growing fastest between 14 and 16 °C and not growing above 20 °C. Fastest growth was found at neutral pH (pH 7.2–7.4) and at marine concentrations of NaCl (20–30 g l −1 ). Phylogenetic analysis of 16S rRNA gene sequences revealed that strain JHA1 T was a member of the family Desulfobacteraceae in the Deltaproteobacteria . The isolate shared 99 % 16S rRNA gene sequence similarity with an environmental sequence obtained from permanently cold Antarctic sediment. The closest recognized relatives were Desulfobacula phenolica DSM 3384 T and Desulfobacula toluolica DSM 7467 T (both 〈 95 % sequence similarity). In contrast to its closest phylogenetic relatives, strain JHA1 T grew chemolithoautotrophically with hydrogen as an electron donor. CO dehydrogenase activity indicated the operation of the reductive acetyl-CoA pathway for inorganic carbon assimilation. Beside differences in physiology and morphology, strain JHA1 T could be distinguished chemotaxonomically from the genus Desulfobacula by the absence of the cellular fatty acid C 16 : 0 10-methyl. Phylogenetic differentiation from other genera was further supported by DsrAB and AprBA sequence analysis. Based on the described phylogenetic and phenotypic differences between strain JHA1 T and its closest relatives, the establishment of a novel genus and a novel species, Desulfoconvexum algidum gen. nov., sp. nov. is proposed. The type strain is JHA1 T ( = DSM 21856 T = JCM 16085 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.043703-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
