In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_5 ( 2014-05-01), p. 1712-1717
Abstract:
An alkaliphilic and halotolerant Gram-stain-positive bacterium, which was isolated from sediment samples from the South China Sea, was subjected to a taxonomic study. The isolate, strain L1 T , grew well at a wide range of temperatures and pH values, 10.0–45.0 °C and pH 6–11, with optima at 30 °C and pH 9.0, respectively. The growth of strain L1 T occurred at total salt concentrations of 0–10 % (w/v) with an optimum at 2 % (w/v). Phylogenetic analysis based on 16S rRNA sequence comparison indicated that the isolate represented a member of the genus Bacillus . The strains most closely related to strain L1 T were Bacillus nanhaiisediminis JCM 16507 T , Bacillus halodurans DSM 497 T and Bacillus pseudofirmus DSM 8715 T , with 16S rRNA similarities of 96.5 %, 95.9 % and 95.7 %, respectively. DNA–DNA hybridization of strain L1 T with the type strains of the most closely related species, B. nanhaiisediminis JCM 16507 T , B. halodurans DSM 497 T and B. pseudofirmus DSM 8715 T , showed reassociation values of about 21.7 %, 14.3 % and 13.9 %, respectively. The DNA G+C content of strain L1 T was 40.76 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained meso -diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids of strain L1 T were iso-C 14 : 0 and anteiso-C 15 : 0 . The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on the phenotypic and phylogenetic characteristics, it is proposed that strain L1 T ( = JCM 18543 T = DSM 26145 T ) should be classified as the type strain of Bacillus ligniniphilus sp. nov.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.058610-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
