In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 57, No. 4 ( 2007-04-01), p. 721-724
Abstract:
A Gram-positive bacterium, strain GW8-1761 T , was isolated from soil close to the Marmore waterfalls, Terni, Italy. 16S rRNA gene sequence similarity studies showed that strain GW8-1761 T belonged to the genus Actinoplanes , being most closely related to Actinoplanes italicus JCM 3165 T (98.9 %), A. rectilineatus IFO 13941 T (98.5 %), A. palleronii JCM 7626 T (97.8 %), A. utahensis IFO 13244 T (97.6 %) and A. cyaneus DSM 46137 T (97.6 %). Strain GW8-1761 T could be distinguished from any other Actinoplanes species with validly published names by 16S rRNA gene sequence similarity values of less than 97.5 %. Chemotaxonomic data [major menaquinone MK-9(H 4 ); major polar lipids diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol, with phosphatidylcholine and aminoglycolipids absent; major fatty acids C 15 : 0 , C 16 : 0 , C 16 : 0 iso, C 17 : 1 ω 8 c and summed feature 3 (C 16 : 1 ω 7 c and/or C 15 : 0 iso 2-OH)] supported the affiliation of strain GW8-1761 T to the genus Actinoplanes . The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW8-1761 T from the most closely related species. Strain GW8-1761 T therefore merits species status, and we propose the name Actinoplanes couchii sp. nov., with the type strain GW8-1761 T (=DSM 45050 T =CIP 109316 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.64805-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2007
detail.hit.zdb_id:
2056611-6
detail.hit.zdb_id:
2002336-4
SSG:
12
