In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 70, No. 4 ( 2020-04-01), p. 2226-2232
Abstract:
A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13 T , was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea. The growth range of NaCl concentration was 0–3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10–40 °C (optimally 28–30 °C), and the pH range for growth was pH 6.0–9.0 (optimally pH 7.0–8.0). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13 T had a high sequence similarity to members of the genus Microbacterium , having the highest similarity with Microbacterium luticocti DSM 19459 T (97.7 %), Microbacterium rhizosphaerae CHO1 T (97.1 %), and Microbacterium immunditiarum SK 18 T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459 T within the genus Microbacterium . A phylogenetic tree based on house-keeping genes also showed the result similar to the 16S rRNA gene-based tree. The main respiratory quinone ( 〉 10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids ( 〉 10 %) were iso -C 16 : 0 , anteiso -C 17 : 0 and anteiso -C 15 : 0 . The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, an inidentified glycolipid and an unidnetified lipid. The peptidoglycan type was supposed to be the B2 ß with amino acids d -alanine, d -glutamic acid, glycine, l -homoserine and d -ornithine. The genomic DNA G+C content was 68.0 mol%. Based on the polyphasic taxonomic data, strain DFW100M-13 T is considered to represent a novel species, for which the name Microbacterium protaetiae sp. nov. is proposed. The type strain is DFW100M-13 T (=KACC 19323 T =NBRC 113120 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.003967
Language:
English
Publisher:
Microbiology Society
Publication Date:
2020
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
