In:
Genes & Development, Cold Spring Harbor Laboratory, Vol. 22, No. 9 ( 2008-05-01), p. 1190-1204
Kurzfassung:
Synthesis of ribosomal RNAs (rRNAs) is the major transcriptional event in proliferating cells. In eukaryotes, ribosomal DNA (rDNA) is transcribed by RNA polymerase I from a multicopy locus coexisting in at least two different chromatin states. This heterogeneity of rDNA chromatin has been an obstacle to defining its molecular composition. We developed an approach to analyze differential protein association with each of the two rDNA chromatin states in vivo in the yeast Saccharomyces cerevisiae . We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1.
Materialart:
Online-Ressource
ISSN:
0890-9369
,
1549-5477
Sprache:
Englisch
Verlag:
Cold Spring Harbor Laboratory
Publikationsdatum:
2008
ZDB Id:
1467414-2
SSG:
12