In:
Genome Research, Cold Spring Harbor Laboratory, Vol. 13, No. 10 ( 2003-10), p. 2348-2352
Abstract:
We used photolithographic microfabrication techniques to create very small stainless steel fountain pens that were installed in place of conventional pens on a microarray spotter. Because of the small feature size produced by the microfabricated pens, we were able to print arrays with up to 25,000 spots/cm 2 , significantly higher than can be achieved by other deposition methods. This feature density is sufficiently large that a standard microscope slide can contain multiple replicates of every gene in a complex organism such as a mouse or human. We tested carryover during array printing with dye solution, labeled DNA, and hybridized DNA, and we found it to be indistinguishable from background. Hybridization also showed good sequence specificity to printed oligonucleotides. In addition to improved slide capacity, the microfabrication process offers the possibility of low-cost mass-produced pens and the flexibility to include novel pen features that cannot be machined with conventional techniques.
Type of Medium:
Online Resource
ISSN:
1088-9051
Language:
English
Publisher:
Cold Spring Harbor Laboratory
Publication Date:
2003
detail.hit.zdb_id:
1483456-X
SSG:
12
