In:
Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory, Vol. 2014, No. 7 ( 2014-07), p. pdb.prot073288-
Abstract:
Fluorescent Ca 2+ indicators are widely used to measure the concentration of free Ca 2+ ([Ca 2+ ] free ) in biological processes. To determine the exact kinetics of changes in [Ca 2+ ] free and the processes underlying these changes (e.g., Ca 2+ binding to Ca 2+ -binding proteins), it is necessary to know the exact binding properties of the indicator used. Here, we describe how to determine the binding rate constants ( k on and k off ) of Ca 2+ indicators.
Type of Medium:
Online Resource
ISSN:
1940-3402
,
1559-6095
DOI:
10.1101/pdb.prot073288
Language:
English
Publisher:
Cold Spring Harbor Laboratory
Publication Date:
2014