In:
Cold Spring Harbor Protocols, Cold Spring Harbor Laboratory, Vol. 2015, No. 12 ( 2015-12), p. pdb.top074443-
Abstract:
Proteomic analysis of early and late endosomes has been constrained by the limited purity of the endosomal fractions that can be achieved by biochemical methods. Here we briefly review endocytic pathways, and then introduce fractionation strategies that have been used to improve the purity of isolated endosomes. In addition, we describe innovative proteomics analysis methods that have been shown to partially circumvent the limitations found in the enrichment steps.
Type of Medium:
Online Resource
ISSN:
1940-3402
,
1559-6095
DOI:
10.1101/pdb.top074443
Language:
English
Publisher:
Cold Spring Harbor Laboratory
Publication Date:
2015