In:
Plant Physiology, Oxford University Press (OUP), Vol. 116, No. 4 ( 1998-04-01), p. 1393-1401
Kurzfassung:
A Cd2+-selective vibrating microelectrode was constructed using a neutral carrier-based Cd ionophore to investigate ion-transport processes along the roots of wheat (Triticum aestivum L.) and two species ofThlaspi, one a Zn/Cd hyperaccumulator and the other a related nonaccumulator. In simple Cd(NO3)2solutions, the electrode exhibited a Nernstian response in solutions with Cd2+ activities as low as 50 nm. Addition of Ca2+ to the calibration solutions did not influence the slope of the calibration curve but reduced the detection limit to a solution activity of 1 μm Cd2+. Addition of high concentrations of K+ and Mg2+ to the calibration solution to mimic the ionic composition of the cytoplasm affected neither the slope nor the sensitivity of the electrode, demonstrating the pH-insensitive electrode's potential for intracellular investigations. The electrode was assayed for selectivity and was shown to be at least 1000 times more selective for Cd2+ than for any of those potentially interfering ions tested. Flux measurements along the roots of the twoThlaspi species showed no differences in the pattern or the magnitude of Cd2+ uptake within the time frame considered. The Cd2+-selective microelectrode will permit detailed investigations of heavy-metal ion transport in plant roots, especially in the area of phytoremediation.
Materialart:
Online-Ressource
ISSN:
1532-2548
,
0032-0889
DOI:
10.1104/pp.116.4.1393
Sprache:
Englisch
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
1998
ZDB Id:
2004346-6
ZDB Id:
208914-2
SSG:
12
