In:
Acta Crystallographica Section D Biological Crystallography, International Union of Crystallography (IUCr), Vol. 62, No. 10 ( 2006-10-01), p. 1232-1242
Abstract:
Structure determination and functional characterization of macromolecular complexes requires the purification of the different subunits in large quantities and their assembly into a functional entity. Although isolation and structure determination of endogenous complexes has been reported, much progress has to be made to make this technology easily accessible. Co-expression of subunits within hosts such as Escherichia coli and insect cells has become more and more amenable, even at the level of high-throughput projects. As part of SPINE (Structural Proteomics In Europe), several laboratories have investigated the use co-expression techniques for their projects, trying to extend from the common binary expression to the more complicated multi-expression systems. A new system for multi-expression in E. coli and a database system dedicated to handle co-expression data are described. Results are also reported from various case studies investigating different methods for performing co-expression in E. coli and insect cells.
Type of Medium:
Online Resource
ISSN:
0907-4449
DOI:
10.1107/S0907444906031003
Language:
Unknown
Publisher:
International Union of Crystallography (IUCr)
Publication Date:
2006
detail.hit.zdb_id:
2968623-4
SSG:
12
SSG:
13