In:
Environmental Microbiology, Wiley, Vol. 17, No. 1 ( 2015-01), p. 91-104
Abstract:
P seudomonas knackmussii B 13 was the first strain to be isolated in 1974 that could degrade chlorinated aromatic hydrocarbons. This discovery was the prologue for subsequent characterization of numerous bacterial metabolic pathways, for genetic and biochemical studies, and which spurred ideas for pollutant bioremediation. In this study, we determined the complete genome sequence of B 13 using next generation sequencing technologies and optical mapping. Genome annotation indicated that B 13 has a variety of metabolic pathways for degrading monoaromatic hydrocarbons including chlorobenzoate, aminophenol, anthranilate and hydroxyquinol, but not polyaromatic compounds. Comparative genome analysis revealed that B 13 is closest to P seudomonas denitrificans and P seudomonas aeruginosa . The B 13 genome contains at least eight genomic islands [prophages and integrative conjugative elements ( ICEs )], which were absent in closely related pseudomonads. We confirm that two ICEs are identical copies of the 103 kb self‐transmissible element ICE clc that carries the genes for chlorocatechol metabolism. Comparison of ICE clc showed that it is composed of a variable and a ‘core’ region, which is very conserved among proteobacterial genomes, suggesting a widely distributed family of so far uncharacterized ICE . Resequencing of two spontaneous B 13 mutants revealed a number of single nucleotide substitutions, as well as excision of a large 220 kb region and a prophage that drastically change the host metabolic capacity and survivability.
Type of Medium:
Online Resource
ISSN:
1462-2912
,
1462-2920
DOI:
10.1111/emi.2015.17.issue-1
DOI:
10.1111/1462-2920.12498
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2020213-1
SSG:
12