In:
Molecular Ecology Resources, Wiley, Vol. 13, No. 3 ( 2013-05), p. 362-368
Abstract:
We report a new approach for molecular sex identification of extant U rsinae and T remarctinae bears. Two Y‐specific fragments ( SMCY and 318.2 ) and one X‐specific fragment ( ZFX ) are amplified in a multiplex PCR, yielding a double test for male‐specific amplification and an internal positive control. The primers were designed and tested to be bear‐specific, thereby minimizing the risk of cross‐amplification in other species including humans. The high sensitivity and small amplicon sizes (100, 124, 160 base pairs) facilitate analysis of non‐invasively obtained DNA material. DNA from tissue and blood as well as from 30 non‐invasively collected hair and faeces yielded clear and easily interpretable results. The fragments were detected both by standard gel electrophoresis and automated capillary electrophoresis.
Type of Medium:
Online Resource
ISSN:
1755-098X
,
1755-0998
DOI:
10.1111/men.2013.13.issue-3
DOI:
10.1111/1755-0998.12072
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2406833-0
SSG:
12