In:
APMIS, Wiley, Vol. 125, No. 10 ( 2017-10), p. 888-901
Abstract:
The study was carried out to study expression of Toll like receptors 2 ( TLR 2), natural/inducible Treg and Interferon‐γ alongside oxidative stress and understand their significance in pediatric samples. Influence of oxidative stress on Celiac Disease was analysed by evaluating lipid peroxidation, reduced glutathione, Glutathione peroxidase, etc. A comparison was performed among CD patients, CD patients on gluten free diet ( GFD ), and healthy controls. Peripheral nT regs exhibited a similar pattern of reduced numbers in CD and GFD cases when compared to healthy controls. On the other hand, inducible Tregs were much lower in GFD patients as compared to CD patients. Expression of TLR 2 on iT regs was elevated in CD and GFD , however, expression on nT regs was unaltered in all the three groups. The inflammatory cytokine IFN ‐γ positive Treg cells were found to be elevated in CD as compared to control group. Oxidative stress was elevated in CD as compared to healthy controls while that in GFD samples was lower in comparison to CD . The levels of LPO , activities of enzymes SOD and Catalase were higher in CD and GFD samples when compared to controls. However, enzyme Glutathione peroxidase and reduced glutathione levels declined in both CD and GFD groups as compared to controls. This report highlights the effect of elevated oxidative stress in CD on reduced traffic of iT regs toward periphery. A strong correlation was observed between the cytokine IFN ‐γ and TLR 2 expression in movement of iT regs in CD patients.
Type of Medium:
Online Resource
ISSN:
0903-4641
,
1600-0463
DOI:
10.1111/apm.2017.125.issue-10
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2098213-6
SSG:
12
