In:
Basic & Clinical Pharmacology & Toxicology, Wiley, Vol. 113, No. 5 ( 2013-11), p. 300-306
Abstract:
Eudesmols are naturally occurring sesquiterpenoid alcohols that present cytotoxic effect to cancer cells. Herein, all eudesmol isomers displayed cytotoxicity to different tumour cell lines. α‐Eudesmol showed IC 50 values ranging from 5.38 ± 1.10 to 10.60 ± 1.33 μg/mL for B 16‐ F 10 and K 562 cell lines, β‐eudesmol showed IC 50 values ranging from 16.51 ± 1.21 to 24.57 ± 2.75 μg/mL for B 16‐ F 10 and H ep G 2 cell lines, and γ‐eudesmol showed IC 50 values ranging from 8.86 ± 1.27 to 15.15 ± 1.06 μg/mL for B 16‐ F 10 and K 562 cell lines, respectively. In addition, in this work, we studied the mechanisms of cytotoxic action of eudesmol isomers (α‐, β‐ and γ‐eudesmol) in human hepatocellular carcinoma H ep G 2 cells. After 24‐hr incubation, H ep G 2 cells treated with eudesmol isomers presented typical hallmarks of apoptosis, as observed by morphological analysis in cells stained with haematoxylin–eosin and acridine orange/ethidium bromide. None of eudesmol isomers caused membrane disruption at any concentration tested. Moreover, eudesmol isomers induced loss of mitochondrial membrane potential and an increase in caspase‐3 activation in H ep G 2 cells, suggesting the induction of caspase‐mediated apoptotic cell death. In conclusion, the eudesmol isomers herein investigated are able to reduce cell proliferation and to induce tumour cell death by caspase‐mediated apoptosis pathways.
Type of Medium:
Online Resource
ISSN:
1742-7835
,
1742-7843
DOI:
10.1111/bcpt.2013.113.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2151592-X
SSG:
15,3
