In:
Clinical Endocrinology, Wiley, Vol. 86, No. 5 ( 2017-05), p. 688-697
Abstract:
This study aimed to explore the role of acute exercise on skeletal muscle gene expression related to insulin resistance in patients with polycystic ovary syndrome ( PCOS ) and controls. Methods Four obese women with PCOS and four body mass index (BMI)‐matched controls (CTRL) participated in this study. After an overnight fast, the subjects underwent a single 40‐min bout of aerobic exercise. Muscle samples were obtained from vastus lateralis at baseline and 60 min after exercise. The expression of a panel of insulin resistance genes was evaluated by a quantitative PCR array system. Network‐based analyses were performed to interpret transcriptional changes occurring before and after the exercise challenge. Results Overall, differentially expressed genes associated with mitochondria function and peroxisome proliferator‐activated receptor signalling were identified. At baseline, there was a significant upregulation of six genes exclusively in PCOS (i.e. NFKBIA , MAPK 3, PPARGC 1A, GAPDH, ACTB and PPARA ). Twelve genes were upregulated in CTRL after a single bout of aerobic exercise (i.e. LEPR , CXCR 4, CCR 5, IL ‐18R1, CRLF 2, ACACA , CEBPA , PPARGC 1A, UCP 1, TNFRSF 1B, TLR 4 and IKBKB ). After the exercise session, three genes were upregulated in PCOS (i.e. SOCS 3, NAMPT and IL ‐8 ), whilst IL ‐6 was upregulated in both groups after exercise. Conclusions This study provides novel evidence on the effects of acute exercise on insulin resistance genes in skeletal muscle of PCOS . The differentially expressed genes reported herein could be further investigated as targets for therapeutic interventions aimed at improving insulin resistance in this syndrome.
Type of Medium:
Online Resource
ISSN:
0300-0664
,
1365-2265
DOI:
10.1111/cen.2017.86.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2004597-9