In:
JDDG: Journal der Deutschen Dermatologischen Gesellschaft, Wiley, Vol. 20, No. 8 ( 2022-08), p. 1112-1121
Abstract:
Onychomycosis (OM) and tinea pedis (TP) are common fungal infections. Currently, diagnosis is based on direct microscopy and culture that have a low to moderate sensitivity and/or require up to 3–4 weeks until results are obtained. PCR techniques have emerged for the diagnosis of fungal infections, but little is known about their sensitivity and specificity in diagnosing. Here, we compared the diagnostic value of a DNA‐chip technology, that detects 56 fungal pathogens, in a single‐center prospective diagnostic study with microscopy and culture in suspected OM/TP. Patients and methods Microscopy, culture and DNA microarray assays were performed on scraping material from patients with suspected OM (n = 67) or TP (n = 73). To test whether swabs can be used as an alternative for scraping, PCR yields were compared in a further 13 patients with OM and 11 patients with TP. Results DNA microarrays had the highest sensitivity. Combination of DNA‐chip technology with microscopy further increased the sensitivity, and results from this combined laboratory diagnosis can be obtained within 24 hours. Comparison of sampling techniques (scraping, dry or wet swab) for DNA‐chip assays showed similar results in suspected OM or TP. Conclusions DNA‐chip technology shows high sensitivity for OM and TP diagnosis, especially when combined with microscopy.
Type of Medium:
Online Resource
ISSN:
1610-0379
,
1610-0387
Language:
German
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
2099463-1