In:
Development, Growth & Differentiation, Wiley, Vol. 56, No. 6 ( 2014-08), p. 469-479
Abstract:
Sry related high mobility group box 17 (Sox17), which is a marker of endodermal cells and a transcriptional regulator, has a critical role in the maintenance of fetal and neonatal hematopoietic stem cells ( HSC ). Sox17 has been identified as a key regulator of the development and differentiation of fetal hematopoietic progenitors from the aorta‐gonad‐mesonephros ( AGM ) region. The co‐culture of Sox17‐transduced hematopoietic progenitor cells ( CD 45 low c‐Kit high cells) from AGM regions on OP 9 stromal cells gives rise to multipotential hematopoietic stem/progenitor cells. Here, we show that in a primary transplantation experiment, Sox17‐transduction in CD 45 low c‐Kit high cells of embryonic day (E) 10.5 AGM increased the absolute number of common myeloid progenitors ( CMP s) in the bone marrow ( BM ) of recipient mice in comparison to that of granulocyte/macrophage progenitors ( GMP s) and the megakaryocyte/erythroid progenitors ( MEP s). When Sox17‐transduced cells were cultured with OP 9 stromal cells, Sox17‐transduced GMP s (Sox17‐ GMP s), Sox17‐transduced CMP s (Sox17‐ CMP s), and Sox17‐transduced MEP s (Sox17‐ MEP s) were generated. Sox17‐ GMP s and Sox17‐ CMP s maintained their self‐renewal capacity and the hematopoietic ability upon co‐culture with the OP 9 stromal cells for some passages. Moreover, Sox17‐ GMP s exhibited the increase in expression of c‐Mpl and GATA ‐2 in comparison to GMP s of BM and Sox17‐ CMP s showed the increase in expression of c‐Mpl, NF ‐E2, and β ‐globin genes in comparison to CMP s of BM . Furthermore, when Sox17‐transduced cells were cultured in methylcellulose to examine the colony‐forming ability, Sox17‐ GMP s and Sox17‐ CMP s maintained the formation of mixed colonies for some passages. Taken together, Sox17 is suggested to regulate the maintenance and differentiation of hematopoietic progenitors derived from AGM regions at midgestation, in particular myeloid progenitors.
Type of Medium:
Online Resource
ISSN:
0012-1592
,
1440-169X
DOI:
10.1111/dgd.2014.56.issue-6
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2020067-5
SSG:
12
