In:
Development, Growth & Differentiation, Wiley, Vol. 61, No. 7-8 ( 2019-09), p. 410-418
Abstract:
Macular fibrosis is a vital obstacle of vision acuity improvement of age‐related macular degeneration patients. This study was to investigate the effects of interleukin 2 ( IL ‐2) on epithelial‐mesenchymal transition ( EMT ), extracellular matrix ( ECM ) synthesis and transforming growth factor β2 ( TGF ‐β2) expression in retinal pigment epithelial ( RPE ) cells. 10 μg/L IL ‐2 was used to induce fibrosis in RPE cells for various times. Western blot was used to detect the EMT marker α‐smooth muscle actin (α‐ SMA ), ECM markers fibronectin (Fn) and type 1 collagen ( COL ‐1), TGF ‐β2, and the activation of the JAK / STAT 3 and NF ‐κB signaling pathway. Furthermore, JAK / STAT 3 and NF ‐κB signaling pathways were specifically blocked by WP 1066 or BAY 11‐7082, respectively, and the expression of α‐ SMA , COL ‐1, Fn and TGF ‐β2 protein were detected. Wound healing and Transwell assays were used to measure cell migration ability of IL ‐2 with or without WP 1066 or BAY 11‐7082. After induction of IL ‐2, the expressions of Fn, COL ‐1, TGF ‐β2 protein were significantly increased, and this effect was correlated with IL ‐2 treatment duration, while α‐ SMA protein expression did not change significantly. Both WP 1066 and BAY 11‐7082 could effectively downregulate the expression of Fn, COL ‐1 and TGF ‐β2 induced by IL ‐2. What's more, both NF ‐κB and JAK / STAT 3 inhibitors could suppress the activation of the other signaling pathway. Additionally, JAK / STAT 3 inhibitor WP 1066 and NF ‐κB inhibitor BAY 11‐7082 could obviously decrease RPE cells migration capability induced by IL ‐2. IL ‐2 promotes cell migration, ECM synthesis and TGF ‐β2 expression in RPE cells via JAK / STAT 3 and NF ‐κB signaling pathways, which may play an important role in proliferative vitreoretinopathy.
Type of Medium:
Online Resource
ISSN:
0012-1592
,
1440-169X
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2020067-5
SSG:
12
