In:
Helicobacter, Wiley, Vol. 24, No. 2 ( 2019-04)
Kurzfassung:
Culture of Helicobacter pylori with previous eradication failure has been emphasized in clinical guidelines. The current unmet need to manage previously treated H pylori is one tool with diagnostic accuracy and ability for antibiotics susceptibility. Gastric juice PCR can provide diagnosis and antibiotics susceptibility; however, whether treatment failure affects its accuracy remains uninvestigated. Our study aimed to investigate diagnostic accuracy and antibiotics susceptibility of juice PCR in previously treated H pylori and to compare with the current standard of culture. Methods We categorized all 547 patients into treatment‐naïve, post‐1st treatment, post‐2nd treatment, and post‐3rd treatment. Helicobacter pylori infection was confirmed using gold standards. Sensitivity, specificity, positive predictive value, negative predictive value, receiver operating characteristic (ROC) curve and area under ROC curve (AUC) of juice PCR and culture were calculated. Intra‐gastric H pylori density was evaluated. Lastly, the antibiotics susceptibility results of gastric juice and culture were compared. Results Our findings demonstrated AUC was higher in juice PCR than culture in all patients (96.7% vs 91.3%, P 〈 0.0001). The superiority of juice PCR was statistically significant in previously treated patients ( P 〈 0.0001) but not in treatment‐naïve patients ( P = 0.13). Antral H pylori density was less marked in previously treated patients ( P = 0.014). The comparisons of PCR‐RFLP and E ‐test for Clarithromycin resistance showed reliable AUC = 89.8%. Conclusion Compared with the current standard of culture, the gastric juice PCR contains the strengths of performing the antibiotics susceptibility and overcomes the shortcomings of low accuracy. Consequently, gastric juice PCR suits the unmet need to manage previously treated H pylori.
Materialart:
Online-Ressource
ISSN:
1083-4389
,
1523-5378
DOI:
10.1111/hel.2019.24.issue-2
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2019
ZDB Id:
2020336-6
SSG:
12
