In:
Histopathology, Wiley, Vol. 65, No. 5 ( 2014-11), p. 613-622
Abstract:
Nuclear relocation of STAT 6 has been shown in tumours with NAB 2– STAT 6 fusion, and has been proposed as an ancillary marker for the diagnosis of solitary fibrous tumours ( SFT s). The aim of this study was to verify the utility of STAT 6 immunohistology in diagnosing SFT . Methods and results A total of 689 formalin‐fixed paraffin‐embedded tumours comprising 35 pleural SFT s and 654 other mesenchymal tumours were investigated for STAT 6 expression using immunohistochemistry. Nine dedifferentiated liposarcomas ( DDLS s) and five SFT s were also examined for the presence of NAB 2 – STAT 6 fusion at the protein level using the proximity ligation assay ( PLA ), and for copy number variants ( CNV s) with the Illumina Infinium Human Methylation450 array. Thirty‐four of 35 SFT s showed strong nuclear STAT 6 expression. Furthermore, five of 68 DDLS s, two of 130 undifferentiated pleomorphic sarcomas and one of 63 cases of nodular fasciitis showed moderate to strong nuclear STAT 6 expression. The PLA indicated the presence of NAB 2– STAT 6 fusion protein in SFT s, but signal was also detected in some DDLS s. Copy number analysis showed an overall low frequency of chromosomal imbalances in SFT s, but complex karyotypes in DDLS s, including amplification of STAT 6 and MDM 2 loci. Conclusions The detection of nuclear relocation of STAT 6 with immunohistochemistry is a characteristic of SFT s, and may serve as a diagnostic marker that indicates NAB 2 – STAT 6 fusion and helps to discriminate SFT s from histological mimics.
Type of Medium:
Online Resource
ISSN:
0309-0167
,
1365-2559
DOI:
10.1111/his.2014.65.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2006447-0