In:
Histopathology, Wiley, Vol. 68, No. 7 ( 2016-06), p. 1055-1062
Abstract:
Polymorphous low‐grade adenocarcinoma ( PLGA ) is the second most common intra‐oral salivary gland malignancy. The vast majority of PLGA s harbour a PRKD 1 E710D hot‐spot somatic mutation or somatic rearrangements of PRKD 1 , PRKD 2 or PRKD 3 . Given the kinase domain homology among PRKD 1 , PRKD 2 and PRKD 3 , we sought to define whether PLGA s lacking PRKD 1 somatic mutations or PRKD gene family rearrangements would be driven by somatic mutations affecting the kinase domains of PRKD 2 or PRKD 3 . Methods and results DNA was extracted from eight microdissected PLGA s lacking PRKD 1 somatic mutations or PRKD gene family rearrangements. Samples were thoroughly centrally reviewed, microdissected and subjected to Sanger sequencing of the kinase domains of the PRKD 2 and PRKD 3 genes. None of the PLGA s lacking PRKD 1 somatic mutations or PRKD gene family rearrangements harboured somatic mutations in the kinase domains of the PRKD 2 or PRKD 3 genes. Conclusion PLGA s lacking PRKD 1 somatic mutations or PRKD gene family rearrangements are unlikely to harbour somatic mutations in the kinase domains of PRKD 2 or PRKD 3 . Further studies are warranted to define the driver genetic events in this subgroup of PLGA s.
Type of Medium:
Online Resource
ISSN:
0309-0167
,
1365-2559
DOI:
10.1111/his.2016.68.issue-7
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2006447-0