In:
International Journal of Food Science & Technology, Wiley, Vol. 49, No. 4 ( 2014-04), p. 1001-1011
Abstract:
Novozym‐435‐catalysed esterification of caprylic acid, capric acid and oleic acid with glycerol for the synthesis of medium‐ and long‐chain triglycerides ( MLCT ) in vacuum and solvent‐free system was investigated in this study. Response surface methodology with a three‐level, four‐factorial design was applied to optimise the enzymatic esterification for the synthesis of MLCT . The optimum conditions were as follows: reaction temperature 90 °C, 4.80 wt% enzyme load (relative to the weight of total substrates) and substrate molar ratio (fatty acids/glycerol) of 3:1 and 12.37 h. Under above‐mentioned conditions, Triglycerides ( TG ) yield, MLCT and the residual free fatty acids ( FFA ) content in the product were 93.54%, 72.19% and 4.21%, respectively. The content of caprylic acid, capric acid and long‐chain fatty acids of TG was 24%, 10% and 66%, respectively. Novozym 435 in the study showed no selectivity for the different fatty acids and also could be used 14 times without obvious loss of enzyme activity.
Type of Medium:
Online Resource
ISSN:
0950-5423
,
1365-2621
DOI:
10.1111/ijfs.2014.49.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2016518-3
detail.hit.zdb_id:
2401430-8