In:
British Journal of Haematology, Wiley, Vol. 76, No. 1 ( 1990-09), p. 12-20
Abstract:
S ummary . Autologous or allogeneic peripheral blood T cells can stimulate the human megakaryocyte progenitor cell (CFU‐Meg)‐derived colony formation in a dose‐dependent fashion in agar cultures of nonadherent (NA), T cell‐depleted (NT) bone marrow (BM) cells. Low concentrations of monocytes and T cells can collaborate in the stimulation of CFU‐Meg colony formation or in the production of megakaryocyte colony stimulating factor (Meg‐CSF) by T cells in the presence of mitogens or IL‐2. Monocytes alone can produce only negligible Meg‐CSF under any conditions. When monocyte conditioned medium (CM) was added to T cell‐stimulated NA, NT BM cell cultures, CFU‐Meg colony growth was appreciably increased compared with that stimulated by T cells alone. Dose‐dependent increase in CFU‐Meg colony growth was noted when varying concentrations of IL‐1 were added to T cell‐stimulated NA, NT cell cultures, although IL,‐1 itself could support no CFU‐Meg colony growth in the absence of T cells. These data suggest that a synergistic interaction between T cells and monocytes during the production of Meg‐CSF by T cells could be partly mediated by IL‐1. IL‐2 was found to stimulate Meg‐CSF production by T cells in the presence or absence of mitogens. IL‐2‐stimulated Meg‐CSF production by T cells was augmented by the addition of monocytes. Although IL‐2 itself had no stimulatory effect on CFU‐Meg colony growth, dramatic augmentation in the CFU‐Meg colony number was noted when IL‐2 was added to T cell‐stimulated NA, NT cell cultures. High concentrations of monocytes and prostaglandin E (PGE) inhibited the CFU‐Meg colony formation. These results suggest that IL‐1 and IL‐2 may play a stimulatory role on the normal human in vitro megakaryocytopoiesis and may be involved in the development of reactive thrombocytosis and bone marrow mega‐karyocytic hyperplasia in various inflammatory diseases.
Type of Medium:
Online Resource
ISSN:
0007-1048
,
1365-2141
DOI:
10.1111/bjh.1990.76.issue-1
DOI:
10.1111/j.1365-2141.1990.tb07830.x
Language:
English
Publisher:
Wiley
Publication Date:
1990
detail.hit.zdb_id:
1475751-5
