In:
Histopathology, Wiley, Vol. 57, No. 2 ( 2010-08), p. 212-219
Abstract:
Kwok T C, Rakha E A, Lee A H S, Grainge M, Green A R, Ellis I O & Powe D G
(2010) Histopathology 57 , 212–219 Histological grading of breast cancer on needle core biopsy: the role of immunohistochemical assessment of proliferation Aims: Histological grade assessed on needle core biopsy (NCB) moderately concurs with the grade in the surgical excision specimen (SES) (κ‐values between 0.35 and 0.65). A major cause of the discrepancy is underestimation of mitoses in the NCB specimen. The aim was to determine the best method of assessing proliferation on NCB. Methods and results: Proliferative activity of 101 invasive carcinomas of the breast on NCB and SES was assessed using mitotic counts on routine haematoxylin and eosin (H & E) sections and immunohistochemical markers Mib‐1 and phosphorylated histone H3 (PPH3). H & E mitotic count in SES was considered as the gold standard. H & E mitotic count was found to be underestimated on NCB when compared with that in SES ( P 〈 0.001), but no significant difference was detected between NCB and SES regarding Mib‐1 ( P = 0.13) or PPH3 ( P = 0.073). Using receiver–operating characteristic curve, Mib‐1 on NCB was found to agree with the gold standard significantly better than routine H & E on NCB. Conclusions: Immunohistochemical markers in NCB showed better concordance with H & E mitotic count in SES (gold standard) than routine H & E mitotic count in NCB. Further refinement of cut‐offs and scoring methods is needed.
Type of Medium:
Online Resource
ISSN:
0309-0167
,
1365-2559
DOI:
10.1111/his.2010.57.issue-2
DOI:
10.1111/j.1365-2559.2010.03620.x
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
2006447-0
detail.hit.zdb_id:
131914-0
