In:
Acta Anaesthesiologica Scandinavica, Wiley, Vol. 51, No. 7 ( 2007-08), p. 893-899
Abstract:
Background: Volatile anesthetics and hypothermia attenuate the inflammatory response. We aimed to compare the anti‐inflammatory effects of sevoflurane and mild hypothermia during experimental endotoxemia in the rat. Methods: Anesthetized, ventilated Sprague–Dawley (SD) rats were randomly treated as follows ( n = 6 per group): lipopolysaccharide (LPS) only, animals received LPS [LPS 5 mg/kg, intravenously (i.v.)] with no further treatment. In the LPS‐hypothermia group, rats were cooled down to a temperature of 33 °C 15 min after LPS‐injection (LPS 5 mg/kg i.v.). In animals of the LPS‐sevoflurane group, sevoflurane inhalation (1 MAC) was initiated 15 min after induction of endotoxemia. The LPS‐sevoflurane‐hypothermia group received combined sevoflurane and hypothermia 15 min after induction of endotoxemia. A Sham group served as control without endotoxemia or treatment. After 4 h of endotoxemia, plasma levels of tumor necrosis factor‐α (TNF‐α), interleukin‐1β (IL‐1β) and IL‐10 were measured. Alveolar macrophages (AM) were ex vivo cultured for nitrite assay. Results: Inhalation of sevoflurane significantly attenuated plasma levels of TNF‐α (–60%, P 〈 0.05) and IL‐1β (–68%, P 〈 0.05) as compared with the LPS‐only group. Hypothermia and its combination with sevoflurane significantly reduced TNF‐α levels (–46% and –58%, each P 〈 0.05), but not IL‐1β. Application of mild hypothermia and also its combination with sevoflurane resulted in a significant increase in plasma IL‐10 as compared with endotoxemic controls. Nitrite release from AM was found to be significantly suppressed by sevoflurane (–83%), hypothermia (–73%) and by the combination of both (–67%) ( P 〈 0.05, each). Conclusion: Our data suggest that sevoflurane and mild hypothermia attenuate the inflammatory response during endotoxemia in vivo thus contributing to their beneficial role in clinical organ protection.
Type of Medium:
Online Resource
ISSN:
0001-5172
,
1399-6576
DOI:
10.1111/aas.2007.51.issue-7
DOI:
10.1111/j.1399-6576.2007.01353.x
Language:
English
Publisher:
Wiley
Publication Date:
2007
detail.hit.zdb_id:
2004319-3
