In:
European Journal of Biochemistry, Wiley, Vol. 164, No. 3 ( 1987-05), p. 687-694
Abstract:
The uncoupling protein (UP) of isolated brown adipose tissue mitochondria was studied with respect to the mechanism of control of UP function by purine nucleotides. Passive transport of H + and Cl − was followed simultaneously in a KCl medium. With both GDP and ATP a higher sensitivity of Cl − transport (apparent K i = 2.2 μM and 4.7 μM respectively) than of H + transport (apparent K i = 7.7 μM and 34 μM respectively) was observed. Chemical modification of isolated mitochondria by diazobenzenesulfonate (DA BS) up to 75 μmol/mg protein did not affect the transport, its ionic selectivity and regulation by endogenous free fatty acids. In contrast, the sensitivity to purine nucleotides of both H + and Cl − translocation was decreased (apparent K i increased 71 and 47 times respectively). DABS decreased the affinity of [ 3 H]GDP for the specific nucleotide‐binding site on mitochondria ( K d increased from 2.7 μM to 13 μM) and depressed, to a smaller extent, the GDP‐binding capacity. Correlation between occupancy of the specific nucleotide‐binding site by GDP and inhibition of transport yielded a linear relationship for Cl − transport in control mitochondria, a biphasic correlation was obtained. The results show that different structural parts of UP are involved in transport and its control by the regulatory ligands and that, in addition to binding of purine nucleotides to UP, the inhibition of ion transport by purine nucleotides depends on an intrinsic factor modulating the inhibitory effect.
Type of Medium:
Online Resource
ISSN:
0014-2956
,
1432-1033
DOI:
10.1111/ejb.1987.164.issue-3
DOI:
10.1111/j.1432-1033.1987.tb11181.x
Language:
English
Publisher:
Wiley
Publication Date:
1987
detail.hit.zdb_id:
1398347-7
detail.hit.zdb_id:
2172518-4
SSG:
12
