In:
Environmental Microbiology, Wiley, Vol. 15, No. 1 ( 2013-01), p. 211-226
Kurzfassung:
Recent research has disclosed a tight connection between obesity, metabolic gut microbial activities and host health. Obtaining a complete understanding of this relationship remains a major goal. Here, we conducted a comparative metagenomic and metaproteomic investigation of gut microbial communities in faecal samples taken from an obese and a lean adolescent. By analysing the diversity of 16S rDNA amplicons (10% operational phylogenetic units being common), 22 Mbp of consensus metagenome sequences (∼ 70% common) and the expression profiles of 613 distinct proteins (82% common), we found that in the obese gut, the total microbiota was more abundant on the phylum Firmicutes (94.6%) as compared with Bacteroidetes (3.2%), although the metabolically active microbiota clearly behaves in a more homogeneous manner with both contributing equally. The lean gut showed a remarkable shift towards Bacteroidetes (18.9% total 16S rDNA), which become the most active fraction (81% proteins). Although the two gut communities maintained largely similar gene repertoires and functional profiles, improved pili‐ and flagella‐mediated host colonization and improved capacity for both complementary aerobic and anaerobic de novo B 12 synthesis, 1,2‐propanediol catabolism (most likely participating in de novo B 12 synthesis) and butyrate production were observed in the obese gut, whereas bacteria from lean gut seem to be more engaged in vitamin B 6 synthesis. Furthermore, this study provides functional evidence that variable combinations of species from different phyla could ‘presumptively’ fulfil overlapping and/or complementary functional roles required by the host, a scenario where minor bacterial taxa seem to be significant active contributors.
Materialart:
Online-Ressource
ISSN:
1462-2912
,
1462-2920
DOI:
10.1111/emi.2013.15.issue-1
DOI:
10.1111/j.1462-2920.2012.02845.x
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2013
ZDB Id:
2020213-1
SSG:
12
