In:
Journal of Neurochemistry, Wiley, Vol. 90, No. 3 ( 2004-08), p. 659-665
Kurzfassung:
The insulin receptor substrate of 53 kDa (IRSp53) is a target of the small GTPase cdc42 which is strongly enriched in the postsynaptic density of excitatory synapses. IRSp53 interacts with the postsynaptic shank1 scaffolding molecule in a cdc42 regulated manner. The functional significance of the cdc42/IRSp53 pathway in postsynaptic sites is however, unclear. Here we identify PSD‐95 as a second synaptic interaction partner of IRSp53. Interaction is mediated by a C‐terminal PDZ binding motif in IRSp53 and the second PDZ domain of PSD‐95. In HEK cells, overexpressed IRSp53 induces filopodia and targets PSD‐95 into these processes. Immunoprecipitation and immunocytochemistry experiments demonstrate that the interaction occurs at postsynaptic sites in the brain. By virtue of its PDZ‐binding and SH3 domains, IRSp53 is capable of inducing the formation of a triple complex (shank1/IRSp53/PSD‐95).
Materialart:
Online-Ressource
ISSN:
0022-3042
,
1471-4159
DOI:
10.1111/jnc.2004.90.issue-3
DOI:
10.1111/j.1471-4159.2004.02523.x
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2004
ZDB Id:
2020528-4
SSG:
12
