In:
Journal of Forensic Sciences, Wiley, Vol. 54, No. 2 ( 2009-03), p. 305-319
Kurzfassung:
Abstract: The Quantifiler ® Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real‐time PCR well. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. A shift in the cycle threshold ( C T ) values for the internal positive control monitors the presence of PCR inhibitors in a sample. The assay is human specific and exhibits a high dynamic range from 0.023 to 50 ng/μL. In addition, the multiplex assay can detect as little as 25 pg/μL of human male DNA in the presence of a 1000‐fold excess of human female DNA. The multiplex assay provides assessment of the DNA extract and guidance for the selection of the appropriate AmpFℓSTR ® Amplification Kit to obtain interpretable short tandem repeat profiles.
Materialart:
Online-Ressource
ISSN:
0022-1198
,
1556-4029
DOI:
10.1111/jfo.2009.54.issue-2
DOI:
10.1111/j.1556-4029.2008.00951.x
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2009
ZDB Id:
2026357-0
SSG:
2,1
