In:
Journal of Forensic Sciences, Wiley, Vol. 54, No. 4 ( 2009-07), p. 953-960
Abstract:
Abstract: The abuse of antibiotic drugs during animal production remains a worldwide problem and the subsequent detection of the residues of various drugs present at low concentrations in complex biological matrices poses significant analytical challenges. The present study outlines a practical biochip assay system to identify antibiotic residues in different animal tissue extracts. The system uses a simple but efficient multiresidue sample extraction procedure to isolate the antibiotic residues which were then identified directly using high‐affinity monoclonal antibodies presented in a competitive immunoassay with conjugated antibiotic hapten‐chips. The hapten‐chip can analyze six samples each for eight antibiotics on a single chip within 3 h. The analytical results with both artificial positive standard samples and the incurred samples show that the antibody hapten‐chip system has a comparable accuracy and a similar sensitivity to a standard ultra performance liquid chromatography–mass spectrometry (MS)/MS assay. In conclusion, an effective analytical screening system based on antibody hapten‐chip was developed for detecting multiple antibiotic residues from multiple samples.
Type of Medium:
Online Resource
ISSN:
0022-1198
,
1556-4029
DOI:
10.1111/jfo.2009.54.issue-4
DOI:
10.1111/j.1556-4029.2009.01049.x
Language:
English
Publisher:
Wiley
Publication Date:
2009
detail.hit.zdb_id:
2026357-0
SSG:
2,1
