In:
European Journal of Oral Sciences, Wiley, Vol. 117, No. 5 ( 2009-10), p. 498-505
Abstract:
Salivary gland epithelial cells (SGECs) have been shown to participate in immunological responses and have been implicated in the pathogenesis of Sjögren’s syndrome (SS). Experimental evidence from animal models indicates that estrogen deficiency may also participate in SS pathogenesis. However, the expression and functionality of the estrogen receptors alpha (ERα) and beta (ERβ) in normal human salivary epithelium is unknown. To investigate these points, formalin‐fixed, paraffin‐embedded specimens and cultured non‐neoplastic SGEC lines derived from nine minor salivary gland (MSG) biopsies with normal histology were studied. Immunohistochemical analyses detected the epithelial expression of ERα, ERβ1, and ERβ2 protein isoforms both in MSG tissues and in cultured SGECs. Such epithelial expression was verified by immunoblotting of various ER proteins in cellular extracts of cultured SGECs (full‐length‐ERα, ERα‐Δ3, ERβ1‐long, ERβ1‐short, and ERβ2‐long isoforms). Estrogens did not induce growth or apoptosis in cultured SGECs. However, similarly to other cellular systems, treatment of cultured SGECs with estrogens (17β‐estradiol and the ERα‐ and ERβ‐selective agonists propylpyrazole‐triol and diarylpropiolnitrile, respectively) inhibited the interferon‐γ‐inducible expression of intercellular adhesion molecule‐1. This finding corroborated the functionality of ER expressed by SGEC. Our results suggest that salivary epithelium expresses constitutively functional ERα and ERβ proteins that apparently mediate immunomodulatory effects.
Type of Medium:
Online Resource
ISSN:
0909-8836
,
1600-0722
DOI:
10.1111/eos.2009.117.issue-5
DOI:
10.1111/j.1600-0722.2009.00659.x
Language:
English
Publisher:
Wiley
Publication Date:
2009
detail.hit.zdb_id:
2025657-7