In:
Basic & Clinical Pharmacology & Toxicology, Wiley, Vol. 111, No. 4 ( 2012-10), p. 248-253
Kurzfassung:
Soybean isoflavone ( SIF ) has been demonstrated to have neuroprotective effects induced by β‐amyloid peptides ( A β) through suppressing oxidative stress; however, the explicit mechanisms still remain uncovered. In the present study, 32 Wistar rats were randomly divided into four groups: an A β1‐42‐treated group, a SIF + A β1‐42 group, a SIF ‐treated group and a control group. We measured the protein content of 8‐hydroxydeoxyguanosine (8‐ O hd G ) and m RNA expression of 8‐oxoguanine DNA glycosylase ( OGG 1). The protein expression of OGG 1, B cl‐xl, B ad, beta subunit of ATP synthase ( ATPB ) and pyruvate dehydrogenase ( PDH ) in brain was also measured. The results showed that the level of 8‐ OH d G in both SIF groups was significantly decreased compared to the A β1‐42‐treated group ( p 〈 0.05), while the m RNA and protein expression of OGG 1 in the SIF + A β1‐42 groups were up‐regulated compared with the A β1‐42‐treated groups ( p 〈 0.05). The expression of B cl‐xl was up‐regulated in the SIF ‐treated group compared with the A β1‐42‐treated groups ( p 〈 0.05), while the expression of B ad was down‐regulated in the two SIF‐treated groups ( p 〈 0.05). A β1‐42 significantly down‐regulated the expression of ATP ase and PDH proteins compared with the control group ( p 〈 0.05). SIF reversed the down‐regulation effects on the mitochondrial energy metabolic enzymes induced by A β1‐42 ( p 〈 0.05) in the rats. These results suggest that SIF alleviate the oxidative stress in neurons and mitochondria of rat brains mediated by A β1‐42, and these protective effects might be associated with the regulation of OGG 1, B ad, B cl‐xl, ATPB and PDH .
Materialart:
Online-Ressource
ISSN:
1742-7835
,
1742-7843
DOI:
10.1111/bcpt.2012.111.issue-4
DOI:
10.1111/j.1742-7843.2012.00900.x
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2012
ZDB Id:
2151592-X
SSG:
15,3
