In:
Acta Ophthalmologica, Wiley, Vol. 100, No. S267 ( 2022-01)
Abstract:
Disturbed proteostasis and protein aggregation have a key role in Alzheimer´s disease and age‐related macular degeneration pathology. In this study, we assessed the electrophysiological and protein aggregation related biomarker changes in 5xFAD mouse model. Methods Scotopic and photopic electroretinograms (ERG) were recorded at 3, 6, 9 and 12‐ months old animals followed by optical coherent tomography (OCT) (WT; n = 12, 5xFAD; n = 12). The eyes of 3‐ and 6‐month‐old WT ( n = 5) and 5xFAD ( n = 5) were used in immunohistochemistry. The paraffin sections were immunostained with the key regulators of lysosomal and proteasomal clearance. Results 5xFAD mice showed decreased scotopic and photopic ERG signaling over time compared to WT mice. However, there were no clear changes in OCT between groups. Immunohistochemistry results revealed that proteasomal clearance was disturbed in 6‐month‐old 5xFAD mice compared to WT. Interestingly, there were no changes in autophagy biomarkers. Conclusions 5xFAD mouse is a good model to study visual function and protein aggregation mechanism in response to degenerative retina processes.
Type of Medium:
Online Resource
ISSN:
1755-375X
,
1755-3768
DOI:
10.1111/aos.v100.s267
DOI:
10.1111/j.1755-3768.2022.009
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
2466981-7
