In:
Journal of Pharmacy and Pharmacology, Oxford University Press (OUP), Vol. 63, No. 9 ( 2011-08-09), p. 1211-1218
Kurzfassung:
To elucidate a novel anti-inflammatory mechanism of myrrh against lipopolysaccharide (LPS)-induced inflammation. Methods RAW264.7 macrophages were cultured in DMEM and then cells were treated with LPS or LPS plus a myrrh methanol extract (MME) for 24 h. The culture medium was collected for determination of nitric oxide (NO), prostaglandin (PG)E2, interleukin (IL)-1β, and tumour necrosis factor (TNF)-α, and cells were harvested by lysis buffer for Western blot analysis. Key findings Our data showed that treatment with the MME (1∼100 µg/ml) did not cause cytotoxicity or activate haem oxygenase-1 (HO-1) protein synthesis in RAW264.7 macrophages. Furthermore, the MME inhibited LPS-stimulated NO, PGE2, IL-1β and TNF-α release and inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 protein expression. Zn(II) protoporphyrin IX, a specific inhibitor of HO-1, blocked the inhibition of iNOS and COX-2 expression by the MME. Conclusions These results suggest that among mechanisms of the anti-inflammatory response, the MME inhibited the production of NO, PGE2, IL-1β and TNF-α by downregulating iNOS and COX-2 gene expression in macrophages and worked through the action of HO-1.
Materialart:
Online-Ressource
ISSN:
0022-3573
,
2042-7158
DOI:
10.1111/j.2042-7158.2011.01329.x
Sprache:
Englisch
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
2011
ZDB Id:
2041988-0
ZDB Id:
2050532-2
SSG:
15,3
