In:
Journal of Integrative Plant Biology, Wiley, Vol. 56, No. 3 ( 2014-03), p. 271-280
Abstract:
To gain a better understanding of the regulation of Zn homeostasis in plants and the degree of conservation of Zn homeostasis between plants and yeast, a cDNA library from the Zn/Cd hyperaccumulating plant species, Noccaea caerulescens , was screened for its ability to restore growth under Zn limiting conditions in the yeast mutant zap1 Δ. ZAP1 is a transcription factor that activates the Zn dependent transcription of yeast genes involved in Zn uptake, including ZRT1 , the yeast high affinity Zn transporter. From this screen two members of the E2F family of transcription factors were found to activate ZRT1 expression in a Zn independent manner. The activation of ZRT1 by the plant E2F proteins involves E2F‐mediated activation of a yeast GATA transcription factor which in turn activates ZRT1 expression. A ZRT1 promoter region necessary for activation by E2F and GATA proteins is upstream of two zinc responsive elements previously shown to bind ZAP1 in ZRT1 . This activation may not involve direct binding of E2F to the ZRT1 promoter. The expression of E2F genes in yeast does not replace function of ZAP1; instead it appears to activate a novel GATA regulatory pathway involved in Zn uptake and homeostasis that is not Zn responsive.
Type of Medium:
Online Resource
ISSN:
1672-9072
,
1744-7909
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2130095-1
SSG:
12
