In:
Journal of Integrative Plant Biology, Wiley, Vol. 65, No. 3 ( 2023-03), p. 633-645
Abstract:
Whole‐genome genotyping methods are important for breeding. However, it has been challenging to develop a robust method for simultaneous foreground and background genotyping that can easily be adapted to different genes and species. In our study, we accidently discovered that in adapter ligation‐mediated PCR, the amplification by primer‐template mismatched annealing (PTMA) along the genome could generate thousands of stable PCR products. Based on this observation, we consequently developed a novel method for simultaneous foreground and background integrated genotyping by sequencing (FBI‐seq) using one specific primer, in which foreground genotyping is performed by primer‐template perfect annealing (PTPA), while background genotyping employs PTMA. Unlike DNA arrays, multiple PCR, or genome target enrichments, FBI‐seq requires little preliminary work for primer design and synthesis, and it is easily adaptable to different foreground genes and species. FBI‐seq therefore provides a prolific, robust, and accurate method for simultaneous foreground and background genotyping to facilitate breeding in the post‐genomics era.
Type of Medium:
Online Resource
ISSN:
1672-9072
,
1744-7909
Language:
English
Publisher:
Wiley
Publication Date:
2023
detail.hit.zdb_id:
2130095-1
SSG:
12