In:
Journal of Oral Pathology & Medicine, Wiley, Vol. 44, No. 10 ( 2015-11), p. 823-830
Abstract:
Lymph node ( LN ) metastasis is the most common cause of oral squamous cell carcinoma ( OSCC )‐related death. Searching the detailed molecular mechanisms involved LN metastasis in OSCC is still an open question. Methods Paired tissue samples from tumor (T) and adjacent non‐cancerous matched tissues ( NCMT ) parts, as well as LN metastatic lesions in patient with OSCC tissues were subjected to quantitative real‐time PCR analysis for the expression levels of Lin28B. Arecoline, a major areca nut alkaloid, was to explore whether expression of Lin28B could be changed dose dependent in oral epithelial cells. Control and Lin28B‐knockdown arecoline‐stimulated oral epithelial cells were subjected to migration/invasion/anchorage‐independent growth assay. Results Compared with NCMT samples from the same OSCC patient, the expression of Lin28B was increased in all of the tumor samples. A similar upregulation of Lin28B was also observed in LN metastatic when compared with local tumors. Arecoline treatment dose dependently induced Lin28B expression in SG and FaDu cells. Lentiviral‐mediated silencing Lin28B expression significantly attenuated arecoline‐induced oncogenicity including proliferation, migration, invasiveness, and anchorage‐independent growth in SG and FaDu cells. Conclusions Lin28B may be a useful biomarker and novel molecular target for LN metastasis OSCC patients' treatment.
Type of Medium:
Online Resource
ISSN:
0904-2512
,
1600-0714
DOI:
10.1111/jop.2015.44.issue-10
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2026385-5
