In:
Microcirculation, Wiley, Vol. 27, No. 4 ( 2020-05)
Abstract:
We aimed to determine whether high‐dose nitroglycerin, a nitric oxide donor, preserves erythrocyte deformability during cardiopulmonary bypass and examines the signaling pathway of nitric oxide in erythrocytes. Methods In a randomized and controlled fashion, forty‐two patients undergoing cardiac surgery with hypothermic cardiopulmonary bypass were allocated to high‐dose (N = 21) and low‐dose groups (N = 21). During rewarming period, patients were given intravenous nitroglycerin with an infusion rate 5 and 1 µg·kg −1 ·min −1 in high‐dose and low‐dose groups, respectively. Tyrosine phosphorylation level of non‐muscle myosin IIA in erythrocyte membrane was used as an index of erythrocyte deformability and analyzed using immunoblotting. Results Tyrosine phosphorylation of non‐muscle myosin IIA was significantly enhanced after bypass in high‐dose group (3.729 ± 1.700 folds, P = .011) but not low‐dose group (1.545 ± 0.595 folds, P = .076). Phosphorylation of aquaporin 1, vasodilator‐stimulated phosphoprotein, and focal adhesion kinase in erythrocyte membrane was also upregulated in high‐dose group after bypass. Besides, plasma nitric oxide level was highly correlated with fold change of non‐muscle myosin IIA phosphorylation (Pearson's correlation coefficient .871). Conclusions High‐dose nitroglycerin administered during cardiopulmonary bypass improves erythrocyte deformability through activating phosphorylation of aquaporin 1, vasodilator‐stimulated phosphoprotein, and focal adhesion kinase in erythrocytes.
Type of Medium:
Online Resource
ISSN:
1073-9688
,
1549-8719
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2008083-9