In:
Molecular Microbiology, Wiley, Vol. 99, No. 5 ( 2016-03), p. 897-908
Kurzfassung:
The genomic pks island codes for the biosynthetic machinery that produces colibactin, a peptide‐polyketide metabolite. Colibactin is a genotoxin that contributes to the virulence of extra‐intestinal pathogenic E scherichia coli and promotes colorectal cancer. In this work, we examined whether the pks ‐encoded clbS gene of unknown function could participate in the self‐protection of E . coli ‐producing colibactin. A clb S mutant was not impaired in the ability to inflict DNA damage in HeL a cells, but the bacteria activated the SOS response and ceased to replicate. This autotoxicity phenotype was markedly enhanced in a clb S uvr B double mutant inactivated for DNA repair by nucleotide excision but was suppressed in a clb S clb A double mutant unable to produce colibactin. In addition, ectopic expression of clb S protected infected HeL a cells from colibactin. Thus, ClbS is a resistance protein blocking the genotoxicity of colibactin both in the procaryotic and the eucaryotic cells.
Materialart:
Online-Ressource
ISSN:
0950-382X
,
1365-2958
DOI:
10.1111/mmi.2016.99.issue-5
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2016
ZDB Id:
1501537-3