In:
New Phytologist, Wiley, Vol. 203, No. 3 ( 2014-08), p. 794-804
Abstract:
Orientation of cell division is critical for plant morphogenesis. This is evident in the formation and function of meristems and for morphogenetic transitions. Mosses undergo such transitions: from two‐dimensional tip‐growing filaments (protonema) to the generation of three‐dimensional leaf‐like structures (gametophores). The Defective Kernel 1 ( DEK 1) protein plays a key role in the perception of and/or response to positional cues that specify the formation and function of the epidermal layer in developing seeds of flowering plants. The moss P hyscomitrella patens contains the highly conserved DEK1 gene. Using efficient gene targeting, we generated a precise PpDEK1 deletion ( ∆dek1 ), which resulted in normal filamentous growth of protonema. Two distinct mutant phenotypes were observed: an excess of buds on the protonema, and abnormal cell divisions in the emerging buds resulting in developmental arrest and the absence of three‐dimensional growth. Overexpression of a complete PpDEK1 cDNA , or the calpain domain of PpDEK1 alone, successfully complements both phenotypes. These results in P . patens demonstrate the morphogenetic importance of the DEK 1 protein in the control of oriented cell divisions. As it is not for protonema, it will allow dissection of the structure/function relationships of the different domains of DEK 1 using gene targeting in null mutant background.
Type of Medium:
Online Resource
ISSN:
0028-646X
,
1469-8137
DOI:
10.1111/nph.2014.203.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
208885-X
detail.hit.zdb_id:
1472194-6
