In:
New Phytologist, Wiley, Vol. 210, No. 4 ( 2016-06), p. 1298-1310
Abstract:
Natural antisense transcripts ( NAT s) are commonly observed in eukaryotic genomes, but only a limited number of such genes have been identified as being involved in gene regulation in plants. In this research, we investigated the function of small RNA derived from a NAT in fiber cell development. Using a map‐based cloning strategy for the first time in tetraploid cotton, we cloned a naked seed mutant gene ( N 1 ) encoding a MYBMIXTA ‐like transcription factor 3 ( MML 3 )/ Gh MYB 25‐like in chromosome A12, Gh MML 3_A12 , that is associated with fuzz fiber development. The extremely low expression of Gh MML 3_A12 in N 1 is associated with NAT production, driven by its 3′ antisense promoter, as indicated by the promoter‐driven histochemical staining assay. In addition, small RNA deep sequencing analysis suggested that the bidirectional transcriptions of Gh MML 3_A12 form double‐stranded RNA s and generate 21–22 nt small RNA s. Therefore, in a fiber‐specific manner, small RNA derived from the Gh MML 3_A12 locus can mediate Gh MML 3_A12 mRNA self‐cleavage and result in the production of naked seeds followed by lint fiber inhibition in N 1 plants. The present research reports the first observation of gene‐mediated NAT s and si RNA directly controlling fiber development in cotton.
Type of Medium:
Online Resource
ISSN:
0028-646X
,
1469-8137
DOI:
10.1111/nph.2016.210.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
208885-X
detail.hit.zdb_id:
1472194-6
