In:
New Phytologist, Wiley, Vol. 218, No. 1 ( 2018-04), p. 253-268
Abstract:
Stomatal immunity restricts bacterial entry to leaves through the recognition of microbe‐associated molecular patterns ( MAMP s) by pattern‐recognition receptors ( PRR s) and downstream abscisic acid and salicylic acid signaling. Through a reverse genetics approach, we characterized the function of the L‐type lectin receptor kinase‐V.2 (Lec RK ‐V.2) and ‐ VII .1 (Lec RK ‐ VII .1). Analyses of interactions with the PRR FLAGELLIN SENSING 2 ( FLS 2) were performed by co‐immunoprecipitation and bimolecular fluorescence complementation and whole‐cell patch‐clamp analyses were used to evaluate guard cell Ca 2+ ‐permeable cation channels. The Arabidopsis thaliana Lec RK ‐V.2 and Lec RK ‐ VII .1 and notably their kinase activities were required for full activation of stomatal immunity. Knockout lecrk‐V.2 and lecrk‐ VII .1 mutants were hyper‐susceptible to Pseudomonas syringae infection and showed defective stomatal closure in response to bacteria or to the MAMP s flagellin and EF ‐Tu. By contrast, Arabidopsis over‐expressing Lec RK ‐V.2 or Lec RK ‐ VII .1 demonstrated a potentiated stomatal immunity. Lec RK ‐V.2 and Lec RK ‐ VII .1 are shown to be part of the FLS 2 PRR complex. In addition, Lec RK ‐V.2 and Lec RK ‐ VII .1 were critical for methyl jasmonate (Me JA )‐mediated stomatal closure, notably for Me JA ‐induced activation of guard cell Ca 2+ ‐permeable cation channels. This study highlights the role of Lec RK ‐V.2 and Lec RK ‐ VII .1 in stomatal immunity at the FLS 2 PRR complex and in Me JA ‐mediated stomatal closure.
Type of Medium:
Online Resource
ISSN:
0028-646X
,
1469-8137
DOI:
10.1111/nph.2018.218.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
208885-X
detail.hit.zdb_id:
1472194-6