In:
Orthopaedic Surgery, Wiley, Vol. 8, No. 2 ( 2016-05), p. 220-225
Abstract:
Giant cell tumor of bone ( GCTB ) invades extensively and metastasizes, however, the pathological grade and imaging findings are not accurate predictors of its prognosis. Thus, the aim of this study was to explore the relationships between expression of cluster of differentiation ( CD) 34 and matrix metalloproteinase‐9 ( MMP ‐9) and the biological behavior of GCTB with the hope of identifying predictors of prognosis. Methods Sixty‐eight patients with GCTBs attending our institution from S eptember 2008 to A ugust 2013 were enrolled in this prospective study and grouped according to tumor location. Relevant patient characteristics were assessed. Additionally, the expression of CD 34 and MM P‐9 in these patients was assayed by an immunohistochemistry staining procedure and the relationships between CD 34/ MMP ‐9 and microvessel density ( MVD ) analyzed by S pearman correlation analysis. Results It was found that CD 34 factor localizes in the cytoplasm of the endothelial cells of small blood vessels in the tumor stroma and is strongly expressed in GCTBs . In addition, radiological grading showed that there was significantly more CD 34 antibody‐labeled MVD in invasive than in non‐invasive tumors ( P 〈 0.05) and significantly more CD 34 antibody‐labeled MVD in patients who developed recurrences than in those who did not ( P 〈 0.05). Expression of MMP ‐9 was localized in the cytoplasm of tumor cells and the rate of MMP ‐9 positivity in GCTBs was significantly higher in active and invasive tumors than in non‐invasive tumors ( P 〈 0.01). Moreover, there were significantly more MVDs in MMP ‐9‐positive than in MMP ‐9 negative tumors ( P 〈 0.01). CD 34 and MMP ‐9 are positively correlated with MVD values in GCTBs and closely correlated with their grade of malignancy. Conclusion Expression of CD 34 and MMP ‐9 accurately predicts clinical behavior detection and prognosis of GCTBs .
Type of Medium:
Online Resource
ISSN:
1757-7853
,
1757-7861
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2483883-4
