In:
Plant Biotechnology Journal, Wiley, Vol. 14, No. 1 ( 2016-01), p. 354-363
Abstract:
Production of novel transgenic floricultural crops with altered petal properties requires transgenes that confer a useful trait and petal‐specific promoters. Several promoters have been shown to control transgenes in petals. However, all suffer from inherent drawbacks such as low petal specificity and restricted activity during the flowering stage. In addition, the promoters were not examined for their ability to confer petal‐specific expression in a wide range of plant species. Here, we report the promoter of In MYB 1 from Japanese morning glory as a novel petal‐specific promoter for molecular breeding of floricultural crops. First, we produced stable In MYB 1 _1kb:: GUS transgenic Arabidopsis and Eustoma plants and characterized spatial and temporal expression patterns under the control of the In MYB 1 promoter by histochemical β ‐glucuronidase ( GUS ) staining. GUS staining patterns were observed only in petals. This result showed that the In MYB 1 promoter functions as a petal‐specific promoter. Second, we transiently introduced the In MYB 1 _1 kb:: GUS construct into Eustoma, chrysanthemum, carnation, Japanese gentian, stock, rose, dendrobium and lily petals by particle bombardment. GUS staining spots were observed in Eustoma, chrysanthemum, carnation, Japanese gentian and stock. These results showed that the In MYB 1 promoter functions in most dicots. Third, to show the In MYB 1 promoter utility in molecular breeding, a MIXTA ‐like gene function was suppressed or enhanced under the control of In MYB 1 promoter in Arabidopsis. The transgenic plant showed a conspicuous morphological change only in the form of wrinkled petals. Based on these results, the In MYB 1 promoter can be used as a petal‐specific promoter in molecular breeding of floricultural crops.
Type of Medium:
Online Resource
ISSN:
1467-7644
,
1467-7652
DOI:
10.1111/pbi.2016.14.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
2136367-5
SSG:
12
