In:
Plant Biotechnology Journal, Wiley, Vol. 17, No. 1 ( 2019-01), p. 164-177
Abstract:
Complex RNA transcription and processing produces a diverse range catalog of long noncoding RNA s (lnc RNA s), important biological regulators that have been implicated in osmotic stress responses in plants. Promoter upstream transcript ( PROMPT ) lnc RNA s share some regulatory elements with the promoters of their neighbouring protein‐coding genes. However, their function remains unknown. Here, using strand‐specific RNA sequencing, we identified 209 differentially regulated osmotic‐responsive PROMPT s in poplar ( Populus simonii ). PROMPT s are transcribed bidirectionally and are more stable than other lnc RNA s. Co‐expression analysis of PROMPT s and protein‐coding genes divided the regulatory network into five independent subnetworks including 27 network modules. Significantly enriched PROMPT s in the network were selected to validate their regulatory roles. We used delaminated layered double hydroxide lactate nanosheets ( LDH ‐lactate‐ NS ) to transport synthetic nucleic acids into live tissues to mimic overexpression and interference of a specific PROMPT . The altered expression of PROMPT _1281 induced the expression of its cis and trans targets, and this interaction was governed by its secondary structure rather than just its primary sequence. Based on this example, we proposed a model that a concentration gradient of PROMPT _1281 is established, which increases the probability of its interaction with targets near its transcription site that shares common motifs. Our results firstly demonstrated that PROMPT _1281 act as carriers of MYB transcription factors to induce the expression of target genes under osmotic stress. In sum, our study identified and validated a set of poplar PROMPT s that likely have regulatory functions in osmotic responses.
Type of Medium:
Online Resource
ISSN:
1467-7644
,
1467-7652
DOI:
10.1111/pbi.2019.17.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2136367-5
SSG:
12
