In:
Pigment Cell & Melanoma Research, Wiley, Vol. 27, No. 2 ( 2014-03), p. 275-286
Abstract:
Expression profiling of micro RNA s in melanoma lesional skin biopsies compared with normal donor skin biopsies, as well as melanoma cell lines compared with normal melanocytes, revealed that hsa‐mi R ‐206 was down‐regulated in melanoma (−75.4‐fold, P = 1.7 × 10 −4 ). Mi R ‐206 has been implicated in a large number of cancers, including breast, lung, colorectal, ovarian, and prostate cancers; however, its role in tumor development remains largely unknown, its biologic function is poorly characterized, and its targets affecting cancer cells are largely unknown. Mi R ‐206 reduced growth and migration/invasion of multiple melanoma cell lines. Bioinformatics identified cell cycle genes CDK 2, CDK 4, C yclin C , and C yclin D 1 as strong candidate targets. Western blots and 3′ UTR reporter gene assays revealed that mi R ‐206 inhibited translation of CDK 4, C yclin D 1, and C yclin C . Additionally, hsa‐mi R ‐206 transfection induced G 1 arrest in multiple melanoma cell lines. These observations support hsa‐mi R ‐206 as a tumor suppressor in melanoma and identify C yclin C , C yclin D 1, and CDK 4 as mi R ‐206 targets.
Type of Medium:
Online Resource
ISSN:
1755-1471
,
1755-148X
DOI:
10.1111/pcmr.2014.27.issue-2
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2425880-5
