In:
Photochemistry and Photobiology, Wiley, Vol. 89, No. 5 ( 2013-09), p. 1064-1070
Abstract:
All‐trans retinoic acid ( ATRA ) plays essential roles in the normal biological processes and the treatment of cancer and skin diseases. Considering its photosensitive property, many studies have been focused on the photochemistry of ATRA . In this study, we investigated the transient phenomena in the laser flash photolysis ( LFP ) of ATRA in microemulsion to further understand the photochemistry of ATRA . Results show that 355 nm LFP of ATRA in both acidic and alkaline conditions leads to the generation of retinoic acid cation radicals ( ATRA •+ ) via biphotonic processes. The employment of microemulsion system allows us to investigate the reaction of hydrophobic ATRA •+ with molecules of different polarity. Therefore, we studied the reaction activity of ATRA •+ to many hydrophobic and hydrophilic molecules. Results show that ATRA •+ can efficiently interact with lysozyme, tyrosine, tryptophan and many antioxidants, such as curcumin (Cur), vitamin C ( VC ) and gallic acid ( GA ). The apparent rate constants of these reactions were measured and compared. These findings suggest that ATRA •+ is a reactive transient product which may pose damage to lysozyme, and antioxidants, such as Cur, VC and GA , may inactivate ATRA •+ by efficient quenching reactions.
Type of Medium:
Online Resource
ISSN:
0031-8655
,
1751-1097
DOI:
10.1111/php.2013.89.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2048860-9
SSG:
12