In:
Photodermatology, Photoimmunology & Photomedicine, Wiley, Vol. 31, No. 3 ( 2015-05), p. 141-148
Kurzfassung:
Recent advances in biomedical optics have enabled dermal and epidermal components to be visualized at subcellular resolution and assessed noninvasively. Multiphoton microscopy ( MPM ) and reflectance confocal microscopy ( RCM ) are noninvasive imaging modalities that have demonstrated promising results in imaging skin micromorphology, and which provide complementary information regarding skin components. This study assesses whether combined MPM / RCM can visualize intracellular and extracellular melanin granules in the epidermis and dermis of normal human skin. Methods We perform MPM and RCM imaging of in vivo and ex vivo skin in the infrared domain. The inherent three‐dimensional optical sectioning capability of MPM / RCM is used to image high‐contrast granular features across skin depths ranging from 50 to 90 μm. The optical images thus obtained were correlated with conventional histologic examination including melanin‐specific staining of ex vivo specimens. Results MPM revealed highly fluorescent granular structures below the dermal–epidermal junction ( DEJ ) region. Histochemical staining also demonstrated melanin‐containing granules that correlate well in size and location with the granular fluorescent structures observed in MPM . Furthermore, the MPM fluorescence excitation wavelength and RCM reflectance of cell culture‐derived melanin were equivalent to those of the granules. Conclusion This study suggests that MPM can noninvasively visualize and quantify subepidermal melanin in situ .
Materialart:
Online-Ressource
ISSN:
0905-4383
,
1600-0781
DOI:
10.1111/phpp.2015.31.issue-3
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2015
ZDB Id:
2026222-X
