In:
The Plant Journal, Wiley, Vol. 114, No. 6 ( 2023-06), p. 1285-1300
Kurzfassung:
Understanding early fruit development in tomato is critical since the size and shape of a fruit is largely decided in the early stages. Our previous work identified hundreds of transcription factors specifically expressed in the pericarp of 0–5 days post anthesis fruits in tomato that are likely involved in this complex process. ASYMMETRIC LEAVES 2 ( AS2 ) belongs to the LATERAL ORGAN BOUNDARIES family, and its role in promoting leaf adaxial cell fates in Arabidopsis has been well characterized. SlAS2 and SlAS2L , two homologs of AS2 in tomato, are specifically expressed in the ovary wall/pericarp at early fruit developmental stage, but their functions in fleshy fruit development are still unclear. In the present study, we found that knockout mutations in SlAS2 or SlAS2L caused significantly reduced pericarp thickness, leading to smaller mature fruit size. Histocytological observation of the pericarp revealed that disruption of SlAS2 or SlAS2L inhibited both cell division and expansion, leading to the observed thinner pericarp. Additionally, leaf and stamen defects in single and double mutants suggested that SlAS2 and SlAS2L have redundant functions in plant development. Both SlAS2 and SlAS2L interact with SlAS1 in vitro , which is consistent with Arabidopsis AS2. Molecular analyses revealed that genes regulated by SlAS2 and SlAS2L in leaf and fruit development are different, and were related to cell division and differentiation processes during tomato pericarp development. Our results confirm the vital roles of SlAS2 and SlAS2L in regulating tomato fruit development.
Materialart:
Online-Ressource
ISSN:
0960-7412
,
1365-313X
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2023
ZDB Id:
2020961-7
SSG:
12
