In:
The Journal of Physiology, Wiley, Vol. 595, No. 10 ( 2017-05-15), p. 3219-3236
Abstract:
The intracellular concentration of free calcium ions ([Ca 2+ ] i ) in a nerve terminal controls both transmitter release and synaptic plasticity. The rapid triggering of transmitter release depends on the local micro‐ or nanodomain of highly elevated [Ca 2+ ] i in the vicinity of open voltage‐gated Ca 2+ channels, whereas short‐term synaptic plasticity is often controlled by global changes in residual [Ca 2+ ] i , averaged over the whole nerve terminal volume. Here we describe dynamic changes of such global [Ca 2+ ] i in the calyx of Held – a giant mammalian glutamatergic nerve terminal, which is particularly suited for biophysical studies. We provide quantitative data on Ca 2+ inflow, Ca 2+ buffering and Ca 2+ clearance. These data allow us to predict changes in [Ca 2+ ] i in the nerve terminal in response to a wide range of stimulus protocols at high temporal resolution and provide a basis for the modelling of short‐term plasticity of glutamatergic synapses.
Type of Medium:
Online Resource
ISSN:
0022-3751
,
1469-7793
DOI:
10.1113/tjp.2017.595.issue-10
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
1475290-6
SSG:
12